Cell Apoptosis models

To assess apoptosis in the cells of the nematode, acridine orange is used. After exposure to the toxicant for 24 h, the nematodes are immersed in mixed medium with acridine orange at 20 °C for 2 h. Then they are placed on top of agar allowing them to recover for 10 min. Finally, they are examined under an inverted fluorescence
microscope with an excitation wavelength of 515 and 488 nm absorption. Apoptotic cells appear yellow or yellow-orange showing increased DNA fragmentation, whereas intact cells are uniformly green.