GFP Reporters models

Transgenic nematodes carrying the GFP gene fused to various stress-inducible gene promoters have been developed for the study of various biochemical pathways. GFP strains are placed in wells containing the sample solutions and suitable controls. The plates are incubated at 20 °C, performing fluorescence readings within 4–6, 8–20,and 24–40 h for short, moderate and long exposures, respectively. GFP expression is quantified using a fluorometer with a wavelength of 485 nm excitation and 525 nm emission. Alternatively, the observation of fluorescence can be achieved under a light microscope, capturing images that are then analyzed by specialized software. The transgenic strain F25B3.3::GFP with fluorescence expression in neurons has been utilized to study heavy metal and pesticide toxicity.