Transgenerational Effects models

Sublethal endpoints such as locomotion and growth can be evaluated in the offspring of exposed parents. Wild-type N2 nematodes at the L3 larval stage are exposed during the time when sperm, ova and eggs begin to form, providing a window of prenatal exposure. Exposed worms are placed on several plates. Some of them are used for measuring the parents after 24 h of exposure, and the others, for obtaining the generations. This assay has been used to assess the effects of antibiotics and heavy metals on the growth and locomotion of exposed parents and their first generation.