Lifespan-SiViS

Lifespan-SiViS

This prototype is the result of the Smartfoods CIEN project (in collaboration with Biopolis S. L.) (2014-2017).

First prototype (2014):

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This prototype is the result of the Smartfoods CIEN project (in collaboration with Biopolis S. L.) (2014-2017).

Second prototype (2016):

 

This prototype is the result of the Smartfoods CIEN project (in collaboration with Biopolis S. L.) (2014-2017).

Final prototype (2020):

 

The final prototype was developed during the CONOCE project (RTI2018-094312-B-I00) (2019-2022).

This device allows the automation of Lifespan, Healthspan and Chemotaxis.

Open hardware:

Assembly description and code of the second prototype is in: https://github.com/JCPuchalt/c-elegans_smartLight

The components and the guidelines to build the final prototype and the assembly description can be found in the repository https://github.com/JCPuchalt/SiViS.

References:

Puchalt, J. C., SanchezSalmerón, A. J., Martorell, P., & Genovés, S. (2019). Active backlight for automating visual monitoring: An analysis of a lighting control technique for Caenorhabditis elegans cultured on standard Petri plates. PloS one, 14(4). https://doi.org/10.1371/journal.pone.0215548 

Puchalt, J. C., Sánchez-Salmerón, A. J., Ivorra, E., Genovés, S.Martínez, R., & Martorell, P. (2020). Improving lifespan automation for Caenorhabditis elegans by using image processing and a post-processing adaptive data filter. Scientific Reports, 10(1), 1-14. https://doi.org/10.1038/s41598-020-65619-4

Puchalt, J. C., Layana Castro, P. E., & Sánchez-Salmerón, A. J. (2020). Reducing Results Variance in Lifespan Machines: An Analysis of the Influence of Vibrotaxis on Wild-Type Caenorhabditis elegans for the Death Criterion. Sensors, 20(21), 5981. https://doi.org/10.3390/s20215981

Puchalt, J. C., Sánchez-Salmerón, A. J., Ivorra, E., Llopis, S., Martínez, R., & Martorell, P. (2021). Small flexible automated system for monitoring Caenorhabditis elegans lifespan based on active vision and image processing techniques. Scientific reports, 11(1), 1-11. https://doi.org/10.1038/s41598-021-91898-6

Lifespan example experiment:

Lifespan 18 Demo ( download images and download results)